《植物生理学报》 2010, 46(6): 597-600

紫外辐射诱导烟草原生质体中 DNA 损伤的彗星电泳检测

张来军^{1, 2}, 郝建国¹, 贾敬芬^1,*

1 西北大学西部资源生物与现代生物技术教育部重点实验室, 西安710069; 2 甘肃省陇东学院生命科学系, 甘肃庆阳745000

通信作者：贾敬芬；E-mail: jiajf38@nwu.edu.cn；Tel: 029-88303484

摘　要：

以烟草原生质体为材料, 采用彗星电泳检测用 0.5 W•m-2 紫外线以不同时间(0、5、10、30、60 和120 s)诱导的烟草原 生质体中 DNA 的损伤。结果表明, 在 0~10 s 的时间内代表 DNA 损伤程度的尾矩、Olive 尾矩等参数与紫外线照射时间具 有良好的时间依赖关系。本文建立的烟草原生质体体系采用彗星电泳技术, 可以快速而灵敏地检测紫外线对植物细胞的损 伤程度。

关键词：彗星电泳; DNA 损伤; UV-B 辐射; 烟草原生质体

收稿：2009-12-10   修定：2010-03-22

资助：国家自然科学基金(30671082)。

Comet Assay on DNA Damage Induced by UV-B Radiation in Tobacco Protoplasts

ZHANG Lai-Jun^1,2, HAO Jian-Guo¹, JIA Jing-Fen^1,*

¹Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China; ²Department of Life Science, Longdong College, Qingyang, Gansu 745000, China

Corresponding author: JIA Jing-Fen; E-mail: jiajf38@nwu.edu.cn; Tel: 029-88303484

Abstract:

Protoplasts of Nicotiana sylvestris was used for experimental material to investigate DNA damage in protoplast which was induced by 0.5 W·cm^-2 UV-B for different time (0, 5, 10, 30, 60, and 120 s) by using comet assay. The results showed that the indexes of tail moment and olive tail moment etc which detected the DNA damage had statistically dose effect relationships with the time of UV-B radiation from 0 to 10 s. Comet assay detected the degree of DNA damage of tobacco protoplast induced by UV-B radiation rapidly and sensitively.

Key words: comet assay; DNA damage; UV-B radiation; tobacco protoplast